Analysis Note

ControlSW1353 cell lysate

Application

Anti-Aggrecan (neoepitope) Antibody, clone BC-3 detects level of Aggrecan (neoepitope) & has been published & validated for use in Western Blotting & IHC.

Research CategoryCell Structure

Western Blot Analysis: A representative lot detected Aggrecan neoepitope in human chondrocite cell lysate.

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Aggrecan (CSPG1, CSPCP, or CSPGCP) is a chondroitin-sulfate proteoglycan belonging to the family of letican proteins. Aggrecan is a large globar structure containing multiple domains including carbohydrate-recognition domains; complement binding protein domains; immunoglobulin domains; proteoglycan tandem repeats; and in some cases, EGF-like domains. It can be degraded by aggrecanase. The N-terminus of aggrecan binds ECM hyaluronan in the presence of the linking protein, and forms complex aggregates, whereas the C-terminus binds a number of other ECM proteins including tenascins, glycolipids, and fibulins. Aggrecan is a major component of cartilage tissue and it enables this tissue to withstand compression by maintaining the viso-elastic properties of cartilage. Aggrecan may also facilitate neural maturation and plasticity by contributing to the formation of perineuronal nets in the developing CNS.

Immunogen

Linear peptide corresponding to human Aggrecan neoepitope.

Epitope: Args neoepitope sequence

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in SW1353 cell lysate.

Western Blot Analysis:1 µg/mL of this antibody detected Aggrecan neoepitope in 10 µg of SW1353 cell lysate.

Specificity

This antibody detectes the Args neoepitope sequence in aggrecanase-cleaved aggrecan (Tortella, M. D., et al. (1999). Science. 284(5420):1664-1666.).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~60 kDa observed. Uniprot describes three isoforms at ~250 kDa, ~246 kDa and ~239 kDa Aggrecan is degraded by aggrecanase into a ~60 kDa protein and other higher molecular weight products (Lemons, M. L. et al. (2001). J Neurosci. 21(13):4772-4781. Forsang, A. J. et al. (1995). Biochem J. 310(Pt 1):337-343.).